detected by western blotting using anti-Flag-HRP and anti-GAPDH antibodies. PVDF membrane was probed with 1 g/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen. Immunoprecipitated proteins were detected by western blotting using rabbit. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. Western blot shows lysates of mouse, human, and rat brain tissue. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). It can recognize the 36 kDa GAPDH protein in most cells/tissues.
Gapdh western blot full#
This antibody is raised against full length GAPDH of human origin. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. (F) Western blots to detect FASN, UCP1, and GAPDH protein in iWAT from the indicated flox/flox control, cAdFASN KO, and cAdMCD KO mice. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. GAPDH is highly conserved across species. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Briefly, the cells were lysed in RIPA buffer containing protease inhibitor and. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. GAPDH is a housekeeping gene and used as controls in both Western blot and qPCR. Western blotting was carried out as described earlier (19).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting.
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